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primary antibodies against p53 (7f5)  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc primary antibodies against p53 (7f5)
    Primary Antibodies Against P53 (7f5), supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+against+p53+(7f5)/p53+primary+antibody/pm40175339-213-8-36
    Average 90 stars, based on 1 article reviews
    primary antibodies against p53 (7f5) - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Membrane:

    Article Title: Window of opportunity trial reveals mechanisms of response and resistance to navtemadlin in patients with recurrent glioblastoma
    Article Snippet: Following protein separation, a dry transfer was completed at 30V for 6 min with PVDF stacks in an iBlot 2 transfer instrument (IB24002, Invitrogen). .. After gel transfer, the membrane was blocked with 5% dry milk (1706404, BioRad) in TBS-T (T1680, Fisher Scientific) for 30 min. Primary incubation with antibodies against rabbit p53 (2527S, Cell Signaling Technology), rabbit PUMA (12450S, Cell Signaling Technology), rabbit CDKN1A/p21 (2947S, Cell Signaling Technology), rabbit MDM2 (86934S, Cell Signaling Technology) and rabbit GAPDH (5174S, Cell Signaling Technology) at a 1:1000 dilution occurred overnight at 4 °C. .. After washes with TBS-T, the membrane was incubated with an anti-rabbit secondary antibody (7074S, Cell Signaling Technology) at a 1:3000 dilution for 1 h at room temperature.

    Article Title: Surgical window of opportunity trial reveals mechanisms of response and resistance to navtemadlin (KRT-232) in patients with recurrent glioblastoma
    Article Snippet: Following protein separation, a dry transfer was completed at 30V for 6 min with PVDF stacks in an iBlot 2 transfer instrument (IB24002, Invitrogen). .. After gel transfer, the membrane was blocked with 5% dry milk (1706404, BioRad) in TBS-T (T1680, Fisher Scientific) for 30 min. Primary incubation with antibodies against rabbit p53 (2527S, Cell Signaling Technology), rabbit PUMA (12450S, Cell Signaling Technology), rabbit CDKN1A/p21 (2947S, Cell Signaling Technology), rabbit MDM2 (86934S, Cell Signaling Technology) and rabbit GAPDH (5174S, Cell Signaling Technology) at a 1:1000 dilution occurred overnight at 4 °C. .. After washes with TBS-T, the membrane was incubated with an anti-rabbit secondary antibody (7074S, Cell Signaling Technology) at a 1:3000 dilution for 1 h at room temperature.

    Incubation:

    Article Title: Window of opportunity trial reveals mechanisms of response and resistance to navtemadlin in patients with recurrent glioblastoma
    Article Snippet: Following protein separation, a dry transfer was completed at 30V for 6 min with PVDF stacks in an iBlot 2 transfer instrument (IB24002, Invitrogen). .. After gel transfer, the membrane was blocked with 5% dry milk (1706404, BioRad) in TBS-T (T1680, Fisher Scientific) for 30 min. Primary incubation with antibodies against rabbit p53 (2527S, Cell Signaling Technology), rabbit PUMA (12450S, Cell Signaling Technology), rabbit CDKN1A/p21 (2947S, Cell Signaling Technology), rabbit MDM2 (86934S, Cell Signaling Technology) and rabbit GAPDH (5174S, Cell Signaling Technology) at a 1:1000 dilution occurred overnight at 4 °C. .. After washes with TBS-T, the membrane was incubated with an anti-rabbit secondary antibody (7074S, Cell Signaling Technology) at a 1:3000 dilution for 1 h at room temperature.

    Article Title: Surgical window of opportunity trial reveals mechanisms of response and resistance to navtemadlin (KRT-232) in patients with recurrent glioblastoma
    Article Snippet: Following protein separation, a dry transfer was completed at 30V for 6 min with PVDF stacks in an iBlot 2 transfer instrument (IB24002, Invitrogen). .. After gel transfer, the membrane was blocked with 5% dry milk (1706404, BioRad) in TBS-T (T1680, Fisher Scientific) for 30 min. Primary incubation with antibodies against rabbit p53 (2527S, Cell Signaling Technology), rabbit PUMA (12450S, Cell Signaling Technology), rabbit CDKN1A/p21 (2947S, Cell Signaling Technology), rabbit MDM2 (86934S, Cell Signaling Technology) and rabbit GAPDH (5174S, Cell Signaling Technology) at a 1:1000 dilution occurred overnight at 4 °C. .. After washes with TBS-T, the membrane was incubated with an anti-rabbit secondary antibody (7074S, Cell Signaling Technology) at a 1:3000 dilution for 1 h at room temperature.

    Article Title: ABCB1 confers resistance to carboplatin by accumulating stem-like cells in the G2/M phase of the cell cycle in p53 null ovarian cancer.
    Article Snippet: In brief, 20 μg of protein was separated and then transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, USA). .. After blocking procedure, membranes were incubated with primary antibodies against p53 (7F5), phospho-p53 (E9Y4U; Ser15), PARP1 (46D11), cleaved-PARP1 (D64E10; Asp214), BCL-XL (54H6), phospho-cdc25c (63F9; Ser216), cyclin B1 (D5C10), ΔNp73 (38C674.2; Novus, USA), and β-actin (all from Cell signaling, USA), respectively, and finally visualized by ECL solution (Thermo, USA). .. Quantification of each protein expression compared to the loading control was conducted by using ImageJ software.

    Article Title: Induction of ferroptosis by brucine suppresses gastric cancer progression through the p53-mediated SLCA711/ALOX12 axis
    Article Snippet: The cells were subsequently incubated for 2 h at room temperature with 5 % bovine serum albumin (BSA, Sigma-Aldrich, St. Louis, USA) after being permeabilized for 20 min with 0.5 % Triton X-100. .. The cells were incubated with a primary antibody against p53 (1:100, 2527, Cell Signaling Technology, Inc., Danvers, USA) at 4 °C for an overnight period after three rounds of washing in phosphate-buffered saline with 0.1 % Tween (PBST). .. After that, the cells underwent treatment for 2 h at room temperature and in the dark with a goat anti-rabbit secondary antibody conjugated with TRITC (1:50, ZF-0317, Beijing Zhongshan Golden Bridge Biotechnology Co., Beijing, China).

    Staining:

    Article Title: Oncolytic virus-mediated p53 activation boosts the antitumor immunity of a p53-transduced dendritic cell vaccine
    Article Snippet: .. Cells were placed on slides and stained overnight with the primary antibody against human p53 (1:800, 2527; Cell Signaling Technology, Beverly, MA, USA). .. After washing with phosphate-buffered saline (PBS), the slides were incubated with Alexa Fluor 647-labeled secondary antibody (1:500, A-21245; Invitrogen, Carlsbad, CA, USA).

    Blocking Assay:

    Article Title: ABCB1 confers resistance to carboplatin by accumulating stem-like cells in the G2/M phase of the cell cycle in p53 null ovarian cancer.
    Article Snippet: In brief, 20 μg of protein was separated and then transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, USA). .. After blocking procedure, membranes were incubated with primary antibodies against p53 (7F5), phospho-p53 (E9Y4U; Ser15), PARP1 (46D11), cleaved-PARP1 (D64E10; Asp214), BCL-XL (54H6), phospho-cdc25c (63F9; Ser216), cyclin B1 (D5C10), ΔNp73 (38C674.2; Novus, USA), and β-actin (all from Cell signaling, USA), respectively, and finally visualized by ECL solution (Thermo, USA). .. Quantification of each protein expression compared to the loading control was conducted by using ImageJ software.

    Saline:

    Article Title: Induction of ferroptosis by brucine suppresses gastric cancer progression through the p53-mediated SLCA711/ALOX12 axis
    Article Snippet: The cells were subsequently incubated for 2 h at room temperature with 5 % bovine serum albumin (BSA, Sigma-Aldrich, St. Louis, USA) after being permeabilized for 20 min with 0.5 % Triton X-100. .. The cells were incubated with a primary antibody against p53 (1:100, 2527, Cell Signaling Technology, Inc., Danvers, USA) at 4 °C for an overnight period after three rounds of washing in phosphate-buffered saline with 0.1 % Tween (PBST). .. After that, the cells underwent treatment for 2 h at room temperature and in the dark with a goat anti-rabbit secondary antibody conjugated with TRITC (1:50, ZF-0317, Beijing Zhongshan Golden Bridge Biotechnology Co., Beijing, China).



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    90
    Cell Signaling Technology Inc primary antibodies against p53 (7f5)
    Primary Antibodies Against P53 (7f5), supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+against+p53+(7f5)/p53+primary+antibody/pm40175339-213-8-36
    Average 90 stars, based on 1 article reviews
    primary antibodies against p53 (7f5) - by Bioz Stars, 2026-09
    90/100 stars
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